Molecular characterization of phytophthora: causal agent of the cocoa brown rot in Cuba

Abstract

Phytophthora’s sorts represent pathogenic devastators’s kind of the vegetables and for resulting music the cause of important economic losses. In Cuba one becomes of the fundamental problems that affect the production of the cocoa. The need of present-day characterization of the fruit’s pathogens, was led to the realization of this work at Lovaina’s Catolic University (Belgium) from September 2010 to March 2011. Were examined Phytophthora’s molecular characteristics, for the sake of going into genetic diversity, utilizing techniques of molecular characterization like the molecular footprint (Fingerprinting M13) and the polymorphism of length of fragments amplified (AFLP). They examined the microorganism’s 90 signs, isolated of Baracoa’s plantations, Guantánamo province, the bigger producer of harvest at the country. The aftermath obtained intervening the program CEQ8000 (Beckman Coulter ), they showed that only 2 ancestries turned out to be of sort tropicalis, the others 88 reciprocated to the sort palmivora, the palmivora found the exclusive presence of the fellow A2 in Phytophthora’s ancestries, that entails to infer the absence of sexual combinations and population’s existence clonal in Cuba. A weak index of genetic diversity without a particular distribution at this locality’s plantations was observed.