Effect of growth regulators on the formation of somatic embryos of the Velasco-5 coffee hybrid
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Abstract
The use of conventional plant growth regulators, such as auxins and synthetic cytokinins, is essential in the somatic embryogenesis of coffee, since their combination stimulates cell dedifferentiation and redifferentiation from plant tissues. We evaluated the effect of different combinations of plant growth regulators on the formation of somatic embryos of the Velasco-5 coffee hybrid. The research was carried out at the Plant Biotechnology Laboratory of the Tercer Frente Agro-Forestal Experimental Station, Santiago de Cuba, during the period from March to July 2021. Different concentrations of naphthaleneacetic acid (NAA) (0.1, 1.0, and 1.5 mg L-1) and Kinetin (0.5, 1.0, and 1.5 mg L-1) were evaluated in the formation of somatic embryos. With all the evaluated combinations of NAA and Kinetin, the formation of somatic embryos of the Velasco-5 coffee hybrid was observed; However, the highest percentage was obtained with the combination of ANA (0.1 mg L-1) and kinetin (0.5 mg L-1). The correct dosage of growth regulators allows optimizing the production of genetically uniform seedlings, which is key to improving crop productivity and resistance.
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