Influencia de concentraciones de Vitrofural en la esterilización de medios de cultivos en la propagación in vitro de Coffea arabica L.
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Abstract
The investigation was developed with the objective of evaluating the influence of different Vitrofural concentrations in the sterilization of cultivations medium in the propagation in the in vitro descendant’s F1 of Coffea arabica L. It was carried out in the vegetable biotechnology laboratory of the Estación Experimental Agro-Forestal Jibacoa, Villa Clara province, Cuba, in the 2017 year. Different Vitrofural concentrations were studied (60 mg • L-1, 80 mg • L-1,100 mg • L-1 and 116 mg • L-1 as control) in the sterilization of cultivations medium semisolids for the calluses formation. The contamination, oxidation, explants was evaluated with call genesis, dry and fresh mass, and the consistency of the explants, carrying out the comparison of its values by means of the test of Tukey and Kruskall Wallis with previous confirmation of the suppositions of normality and variance homogeneity using the statistical package InfoStat, 2012 version 1.0. The Vitrofural concentrations of influenced in the sterilization of cultivations medium in the phase of calluses formation for the in vitro propagation of the descendant’s F1 of Coffea arabica L. The concentration of 80 mg • L-1 turned out to be the most economic without differing of the superior concentrations in the contamination of the explants. The Vitrofural concentrations didn’t influence in the call genesis induction and in the consistency of the explants; however, with 80 and 60 mg • L-1 was increased the dry mass of the calluses significantly.
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